The Physics of Optical Tweezers and Their Biological Applications
Introduction
Optical tweezers, also known as optical traps, are highly focused light beams that are capable of holding and manipulating microscopic particles. The technology was first proposed by Arthur Ashkin in 1970, and has since found a wide range of applications, particularly in the field of biology. This article will delve into the physics behind optical tweezers, and explore their various biological applications.
Physics of Optical Tweezers
Principle of Operation
Optical tweezers operate on the principle of radiation pressure, which refers to the pressure exerted by light when it is absorbed or reflected by a surface. When a highly focused light beam encounters a small particle, it exerts a force on the particle, pushing it towards the region of highest light intensity. This is known as the gradient force.
Creation of Optical Traps
To create an optical trap, a high numerical aperture microscope objective is used to focus a laser beam to a very small spot. The gradient force then traps the particle in the axial direction, while the scattering force, which is the force exerted by the light scattering off the particle, counteracts the gradient force in the radial direction, thereby trapping the particle in three dimensions.
Factors Affecting Trapping Efficiency
The efficiency of an optical trap is influenced by several factors, including the wavelength of the light, the refractive index of the particle and the surrounding medium, and the size and shape of the particle. In general, particles with a higher refractive index than the surrounding medium can be trapped more efficiently.
Biological Applications of Optical Tweezers
Optical tweezers have found a wide range of applications in biology, due to their ability to manipulate microscopic particles without causing physical damage.
Cell Manipulation
One of the most common applications of optical tweezers in biology is cell manipulation. By using optical tweezers, scientists can isolate individual cells from a sample, move them to a new location, and even arrange them in specific patterns. This has proven particularly useful in studies of cell-cell interactions and cell migration.
DNA Manipulation
Optical tweezers can also be used to manipulate DNA molecules. By attaching microscopic beads to the ends of a DNA molecule, scientists can use optical tweezers to stretch the molecule, allowing them to study its mechanical properties and how it interacts with other molecules.
Protein Studies
Another important application of optical tweezers is in the study of proteins. For example, optical tweezers can be used to measure the forces exerted by motor proteins as they move along a microtubule. This can provide valuable insights into the mechanisms of cellular transport.