View source for Base excision repair

==Introduction==
Base excision repair (BER) is a crucial cellular mechanism that rectifies DNA damage instigated by oxidation, deamination, or alkylation. These alterations to the DNA structure can potentially lead to mutations and cell death if not repaired promptly. BER plays a pivotal role in maintaining genomic stability, preventing mutations, and inhibiting the development of numerous human diseases, including [[Cancer|cancer]]<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574798/</ref>.

==Mechanism of Base Excision Repair==
The BER process involves several steps, each facilitated by a specific set of enzymes. The first step is the recognition and removal of the damaged base. This is achieved by a [[DNA glycosylase|DNA glycosylase]], which cleaves the N-glycosidic bond between the base and the sugar phosphate backbone of the DNA molecule, creating an apurinic/apyrimidinic (AP) site<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632168/</ref>.

[[Image:Detail-32108.jpg|thumb|center|A DNA glycosylase enzyme removing a damaged base from the DNA molecule|class=only_on_mobile]]
[[Image:Detail-32109.jpg|thumb|center|A DNA glycosylase enzyme removing a damaged base from the DNA molecule|class=only_on_desktop]]

The AP site is then processed by an AP endonuclease, which cleaves the phosphodiester bond 5' to the AP site, leaving a 3'-hydroxyl group and a 5'-deoxyribose phosphate (dRP) group. This step is followed by the action of a phosphodiesterase, which removes the dRP group<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830716/</ref>.

[[Image:Detail-32110.jpg|thumb|center|An AP endonuclease enzyme cleaving the phosphodiester bond at the AP site|class=only_on_mobile]]
[[Image:Detail-32111.jpg|thumb|center|An AP endonuclease enzyme cleaving the phosphodiester bond at the AP site|class=only_on_desktop]]

The resulting single-nucleotide gap is then filled by a DNA polymerase, which adds the correct nucleotide using the undamaged DNA strand as a template. Finally, the nick in the DNA backbone is sealed by a DNA ligase, restoring the integrity of the DNA molecule<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574798/</ref>.

[[Image:Detail-32112.jpg|thumb|center|A DNA ligase enzyme sealing the nick in the DNA backbone|class=only_on_mobile]]
[[Image:Detail-32113.jpg|thumb|center|A DNA ligase enzyme sealing the nick in the DNA backbone|class=only_on_desktop]]

==Types of Base Excision Repair==
There are two sub-pathways of BER: short-patch BER, which replaces a single nucleotide, and long-patch BER, which replaces 2-10 nucleotides. The choice between these two pathways depends on the type of damage, the cell cycle stage, and the availability of repair proteins<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632168/</ref>.

===Short-Patch BER===
Short-patch BER is the primary pathway for repairing small, non-helix-distorting base lesions. This pathway is initiated by a specific DNA glycosylase, which recognizes and removes the damaged base. The AP site is then processed by an AP endonuclease, followed by the action of a phosphodiesterase. The single-nucleotide gap is filled by DNA polymerase β (Pol β), and the nick in the DNA backbone is sealed by DNA ligase III (Lig III)<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830716/</ref>.

[[Image:Detail-32114.jpg|thumb|center|The process of short-patch BER, showing the removal of the damaged base, the creation of the AP site, and the filling and sealing of the single-nucleotide gap|class=only_on_mobile]]
[[Image:Detail-32115.jpg|thumb|center|The process of short-patch BER, showing the removal of the damaged base, the creation of the AP site, and the filling and sealing of the single-nucleotide gap|class=only_on_desktop]]

===Long-Patch BER===
Long-patch BER repairs larger, helix-distorting lesions and involves the synthesis of a longer DNA patch. This pathway is initiated by the same steps as short-patch BER, but instead of Pol β and Lig III, it involves the action of DNA polymerase δ or ε (Pol δ/ε) and DNA ligase I (Lig I). The displaced DNA strand is then removed by a flap endonuclease<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574798/</ref>.

[[Image:Detail-32116.jpg|thumb|center|The process of long-patch BER, showing the removal of the damaged base, the creation of the AP site, and the synthesis and removal of the longer DNA patch|class=only_on_mobile]]
[[Image:Detail-32117.jpg|thumb|center|The process of long-patch BER, showing the removal of the damaged base, the creation of the AP site, and the synthesis and removal of the longer DNA patch|class=only_on_desktop]]

==Role in Disease Prevention==
BER plays a crucial role in preventing the accumulation of DNA damage and the subsequent development of diseases. Mutations in BER genes can lead to various genetic disorders and increase the risk of cancer. For example, mutations in the gene encoding the DNA glycosylase MUTYH are associated with a hereditary form of colorectal cancer known as [[MUTYH-associated polyposis|MUTYH-associated polyposis]] (MAP)<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632168/</ref>.

==See Also==
* [[DNA replication]]
* [[DNA damage (naturally occurring)]]
* [[Nucleotide excision repair]]
* [[Mismatch repair]]
* [[Direct reversal DNA repair]]

==References==
<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574798/</ref>
<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632168/</ref>
<ref>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830716/</ref>

[[Category:DNA repair]]
[[Category:Genetic diseases]]
[[Category:Cancer biology]]